Inject embryos with:
P(lacw), Plaw or PlwB (red eyes, lac Z and cloning vector)
p
25.7c (transposase,
defective inverted repeats)
The creation of entire new sets of P-elements carrying the w+ genetic marker insertion lines of Drosophila starts with the injection of two transposons into embryos of a white-eyed strain of flies. One of the transposons will be stably inserted into the chromosomes of some of the germline cells of the injected embryos. This transposon is the one from which the transposase gene has been removed and replaced with a genetic marker, a cloning vector and the lac Z gene. The second transposon, known as the helper P-element, contains the complete coding region for transposase, but lacks part of the terminal repeats needed for incorporation of the transposon into the genome. The second transposon is able to produce active transposase in the injected embryos. This allows the other transposon to insert randomly in the genome of germline cells. The transposon does not incorporate into somatic cells due to faulty splicing.
Inject embryos with:
P(lacw), Plaw or PlwB (red eyes, lac Z and cloning vector)
p25.7c (transposase,
defective inverted repeats)