Technique and Skills

1-2 times

frequently

Use of Molecular Databases and Analysis Programs

X

Reporter Gene Expression Analysis

X

Chromosomal DNA Preparation

X

Agarose Gel Electrophoresis

X

Use of Restriction Enzymes

X

Use of Other Modifying enzymes (ligase, Klenow…)

X

Transformation - Electroporation and Heat Shock

X

Visible and UV Spectrophotometry

X

Alkaline Lysis Preparation of Plasmid DNA

X

Commercial Column Preparation of Plasmid DNA

X

Preparation of Total and Poly A+ RNA

X

Denaturing Agarose Gel

X

Northern Blot

X

Southern Blot

X

Non-radioactive Labeling and Detection

X

Hybridization and Washing of Blots and filters

X

PCR Primer Design

X

RT-PCR

X

Screening of Phage Library

X

Construction and Screening of Plasmid Library

X

In vivo excision of plasmid vector from phagemid

X

Subcloning into Expression Vector

X

Expression of Protein in Bacteria

X

Purification of Expressed Protein

X

SDS-PAGE Electrophoresis

X