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SOLUTIONS

The recipes given below are for general use solutions and reagents. Specialized solutions that are used in only one protocol are included within the protocol.

Add to LB or LB agar at concentration of 75µg/ml

Add to LB or LB agar at concentration of 75µg/ml

10 g Ficoll 400
10 g polyvinylpyrrolidone
10 g BSA
H₂O to 500 ml

Filter and store at -20°C in 25 ml aliquots

40 ml grape juice
160 ml water
4 g bacteriological agar

• boil to dissolve agar
• cool to 50°C
• add 1 ml glacial acetic acid and 2 ml 95% ethanol

100mM Tris-HCl, pH 9.1
100mM NaCl
200mM sucrose
50 mm EDTA
0.5% SDS

20% (w/v) Ficoll 400
0.1 M Na₂ EDTA, pH 8.0
1.0% (w/v) SDS
0.25% (w/v) bromphenol blue
0.25% (w/v) xylene cyanol 

10 g tryptone
5 g yeast extract
5 g NaCl
1 ml 1M NaOH

1.5% bacteriological agar in LB broth

80 g NaCl
2 g KCl
11.5 g Na₂HPO₄*7 H₂O
2 g KH₂PO₄

1X PBS with 0.5% Triton-X 100

0.5% yeast extract
2% tryptone
10 mM NaCl
2.5 mM KCl
10 mM MgCl
20 mM MgSO₄
20 mM glucose

242 g Tris base
57.1 ml glacial acetic acid
37.2 g Na₂EDTA-2H₂0
H₂O to 1 liter
TENS Solution