1) Cut open the pouch and remove the gel. Rinse the gel with deionized water.

2) Peel the tape off of the bottom of the cassette.

3) Mount the cassette into the electrophoresis apparatus so that the printed side faces the outer (anode) buffer chamber.

4) Fill the buffer chamber with running buffer.

5) Gently pull the comb out of the cassette. Save the comb so it can be used to separate the cassette plates at the end of the run.

6) Use a pipette to wash the sample wells with 1X running buffer, displacing any bubbles in the wells.

7) Load sample into wells. Fill unused wells with 1X running buffer

8) Attach the electrode apparatus to the power supply and run the gel at a constant voltage 125 V until the dye front is near the bottom of the gels.

9) Remove the gels. Place the cassettes on a flat surface with the notched (well) side facing up. Use the gel comb to separate the plates, starting near the top and working your way around the cassette.

10) Carefully remove the short plate. The gel will adhere to one of the plates. Hold the plate with the gel over an open container. Loosen the gel and place it in the container.

11) Cover the gel with Coomassie Stain. I will allow the gel to stain for 2 hours and then destain gently overnight.